Download e-book for kindle: Advances in lipid research. Volume 7 by Rodolfo Paoletti, Dr. David Kritchevsky

By Rodolfo Paoletti, Dr. David Kritchevsky

ISBN-10: 0120249073

ISBN-13: 9780120249077

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Other histochemical studies of lipid storage diseases have been reviewed by Diezel (1957a,b), Wolman (1964), and Adams (1965d). 4. Pulmonary Physiology and Pathology a. " Pulmonary phospholipid synthesis may play an important role in providing a "surfactant" substance (see Section ΙΙ,Α) that promotes alveolar expansion in the fetal lung (Reynolds and Strang, 1966; Taylor and Abrams, 1966). Histochemical studies have demonstrated a film of phospholipid over the pulmonary epithelial surface. Hitherto, it was tacitly assumed that such phospholipid is synthesized by alveolar macrophages, for phospholipid accumulates in these cells in the cholesterol-fed rabbit (Adams, 1962, unpublished observation) and the capacity to synthesize phospholipid is a common feature of the macrophage elements of the reticuloendothelial system (Day, 1964).

1968). Variants of the basic Linderstr0m-Lang technique have been introduced for the direct thin-layer chromatography of frozen sections from brain (Strich, 1965) and atherosclerotic plaques (Liadsky and Woolf, 1967). The advantage of such histochromatography is that the sections require no preliminary extraction with solvents and are directly chromatogramed after they have been embedded under the silica gel film; their disadvantage is that at present they are essentially nonquantitative. IV.

1966 for further details). Lipase from California Biochemical Corporation is free of contaminating enzymic activity. ] Wash well in several changes of distilled water for 15 minutes. Treat with 1 % lead nitrate for 15 minutes. Wash well in several changes of distilled water. Immerse in dilute ammonium sulfide (10 drops/25 ml) for 1 minute. Wash well, counterstain with Mayer's hemalum, and mount in glycerine jelly. Triglycérides and waxes are stained brownish-black. Only the surface of large fat droplets is stained because the reagents only slowly penetrate such droplets and because lipolysis only proceeds at the water-fat interface.

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Advances in lipid research. Volume 7 by Rodolfo Paoletti, Dr. David Kritchevsky

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