By Anton Posch
This publication provides vast insurance of the rules and up to date advancements of pattern education and fractionation instruments in Expression Proteomics generally and two-dimensional electrophoresis (2-DE) specifically. With its specified ability to unravel millions of proteins in one run, 2-DE continues to be a basic examine software for almost all protein-related medical projects.
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Extra info for 2D PAGE: Sample Preparation and Fractionation
In contrast to urea, substituted ureas are superior for breaking hydrophobic interactions and for improved solubilization of hydrophobic proteins (2,5). Unfortunately, their usefulness is somewhat limited because of their poor solubility in water. Currently, the best solution for solubilization of hydrophobic proteins for 2-DE is a combination of 5–8 M urea and 2 M thiourea (Fig. 1), in combination with appropriate detergents (see Note 1). Fig. 1. Structural formula of urea and thiourea chaotropes.
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2D PAGE: Sample Preparation and Fractionation by Anton Posch